In growth stage.Fatty acid shifts within the 3 growth stagesFatty acid profile was presented in three growth stages of microalgae below the influence of distinctive N-sources (Fig. three). Regarding the fatty acid composition, among the main objectives of this study was to improve DHA (C22:6n3) content in C. cohnii. The highest DHA content was attained in lipid turnover stage (35.7 1.three TFA) having a slight distinction from lipid accumulation stage (34.eight 0.9 TFA) below NaNO3 which was 324 larger than that of NH4HCO3 (Table 1). Equivalent final results have been identified in newly isolated Crypthecodinium sp. SUN, which yielded 34.1 0.three TFA at 120 h and 35.0 0.two TFA at 144 h below light (Sun et al. 2017). For comparison, the general DHA productivity, at the same time as yield (per gram of glucose exhaustion) for the duration of 3 development stages is offered in Table 1. It was apparent that NaNO3 supported drastically greater DHA content material (0.99 0.05 g/L), DHA productivity (193.84 two.76 mg/L day) and DHA yield (44.16 3.64 mg/g glucose) which have been two.1 to two.8-folds larger than other N-sources (P 0.001). When compared with DHA, in development stage palmitic acid (C16:0) was made in the highest percentageFig. two The time-course profile of residual substrates (glucose, nitrate and phosphorus) concentration in C. cohnii cultured on unique nitrogen sources for 7 days. All experiments were performed in triplicate. The information presented here is imply SDSafdar et al. AMB Expr (2017) 7:Web page 6 ofTable 1 Comparison of DHA content material, DHA productivity, lipid and DHA yield of C. cohnii below diverse N-sourcesNsource (NH4)2SO4 Development stage Growth Lipid accumulation Lipid turnover NH4HCO3 Development Lipid accumulation Lipid turnover (NH2)2CO Development Lipid accumulation Lipid turnover NaNO3 Development Lipid accumulation Lipid turnover CDHA (g/L) 0.Formula of β-Aspartylaspartic acid 35 0.Formula of 6-Bromo-2H-benzofuran-3-one 1 0.05 0.0 PDHA (mg/L day) 70.9 eight.9 22.four four.9 Ya (mg/g GLC) L 65.PMID:23695992 six 1.four 35.five 2.a YDHA (mg/g GLC)0.42 0.0.43 0.05 0.06 0.0.39 0.0.05 0.60.three 7.60.7 four.77.three 3.25.eight 1.71.3 five.0.46 0.0.46 0.05 0.13 0.65.three 4.91.1 3.27.five three.66.8 3.74.four 1.28.9 4.19.7 1.16.9 two.3.7 0.All experiments have been performed in triplicate. The data presented here is imply SDa0.99 0.0.97 0.141.1 five.193.8 2.63.four 1.73.4 three.75.1 2.34.3 six.19.1 1.17.6 0.three.9 1.127.1 9.130.3 four.43.1 three.20.6 0.20.7 0.four.two 1.44.1 2.44.1 three.7.4 1.CDHA DHA content, PDHA DHA productivity (g/L day), YL lipid yield (mg/g glucose), YDHA DHA yield (g/g glucose) Yield was calculated on basis of glucose utilizedFig. three Fatty acid profile ( total fatty acid) of C. Cohnii below different N-sources in 3 growth stages; cell development, lipid accumulation and lipid turnover stage, harvested on 48, 120 and 168 h, respectively. All experiments were performed in triplicate. The information presented here is mean SD(27.25.7 TFA) amongst all fatty acids in all treatments. A gradual decline was observed in saturated fatty acids (SFAs) myristic acid (C14:0), C16:0 and stearic acid (C18:0) in all the 4 remedies all through the cultivation time (Fig. three). In contrast, mono-unsaturated fatty acids (MUFAs) palmitoleic acid (C16:1) and oleic acid(C18:1), elevated through lipid accumulation stage and once again decreased in lipid turnover stage in all remedies whilst no substantial change was observed in Linoleic acid (C18:2n6) content ( TFA) (Fig. three). Combined C16C18 content material ( TFA) was calculated as 53.1, 62.5, 59.7, 58.1 in cell development stage and 51.9, 54.7, 53.1, 49.eight inSafdar et al. AMB Expr (2017) 7:Page 7 oflipid accumulation stage beneath (NH4)2SO4, NH4HCO3, (NH2)2CO,.
